{"slip": { "id": 72, "advice": "Don't eat anything your grandparents wouldn't recognise as food."}}
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{"type":"standard","title":"Slice preparation","displaytitle":"Slice preparation","namespace":{"id":0,"text":""},"wikibase_item":"Q7540236","titles":{"canonical":"Slice_preparation","normalized":"Slice preparation","display":"Slice preparation"},"pageid":29765867,"thumbnail":{"source":"https://upload.wikimedia.org/wikipedia/commons/thumb/f/f7/201312_mice_brain_slice_2.png/330px-201312_mice_brain_slice_2.png","width":320,"height":320},"originalimage":{"source":"https://upload.wikimedia.org/wikipedia/commons/f/f7/201312_mice_brain_slice_2.png","width":400,"height":400},"lang":"en","dir":"ltr","revision":"1264951942","tid":"8b8ad4d0-c1d8-11ef-979a-bb9f6d915c1d","timestamp":"2024-12-24T09:22:07Z","description":"Laboratory method involving brain slices","description_source":"local","content_urls":{"desktop":{"page":"https://en.wikipedia.org/wiki/Slice_preparation","revisions":"https://en.wikipedia.org/wiki/Slice_preparation?action=history","edit":"https://en.wikipedia.org/wiki/Slice_preparation?action=edit","talk":"https://en.wikipedia.org/wiki/Talk:Slice_preparation"},"mobile":{"page":"https://en.m.wikipedia.org/wiki/Slice_preparation","revisions":"https://en.m.wikipedia.org/wiki/Special:History/Slice_preparation","edit":"https://en.m.wikipedia.org/wiki/Slice_preparation?action=edit","talk":"https://en.m.wikipedia.org/wiki/Talk:Slice_preparation"}},"extract":"The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or recording. The technique allows for greater experimental control, through elimination of the effects of the rest of the brain on the circuit of interest, careful control of the physiological conditions through perfusion of substrates through the incubation fluid, to precise manipulation of neurotransmitter activity through perfusion of agonists and antagonists. However, the increase in control comes with a decrease in the ease with which the results can be applied to the whole neural system.","extract_html":"
The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or recording. The technique allows for greater experimental control, through elimination of the effects of the rest of the brain on the circuit of interest, careful control of the physiological conditions through perfusion of substrates through the incubation fluid, to precise manipulation of neurotransmitter activity through perfusion of agonists and antagonists. However, the increase in control comes with a decrease in the ease with which the results can be applied to the whole neural system.
"}{"slip": { "id": 195, "advice": "Exercise in the rain can really make you feel alive."}}
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